FastQCFastQC Report
Mon 6 Feb 2017
SRR2079280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5423848
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA136850.043.6013071
GGTATCA49250.038.864711
TATCAAC184700.032.0623862
ATCAACG190550.030.9692633
TCAACGC192500.030.6515854
CAACGCA197700.029.8448225
AACGCAG204200.028.9590056
ACGCAGA232300.025.2301677
CGCAGAG238200.024.5031158
GAGTACT158300.024.09865812
AGAGTAC238550.023.35133711
AGTACTT167450.023.0496113
CAGAGTA253350.022.20504810
GTACTTT172600.022.00215314
GCAGAGT260800.021.9410539
TACTTTT190050.019.9598315
GTACATG175600.019.6755731
ACTTTTT197300.019.40132916
TACATGG179600.018.9295672
ACATGGG187200.017.607373
Produced by FastQC (version 0.11.5)