FastQCFastQC Report
Mon 6 Feb 2017
SRR2079279_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079279_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622671
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39070.24077585659693185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35790.2205622704787354No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAA24940.15369720664262812No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC20040.12350008103922483No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGCGTTCTATCATTCTAGCCTCG18130.11172936473259212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCCG1200.037.3727659
GTATCAA83450.037.1231
CGAACGG709.7253E-734.50644330
GATCGCC400.00259780334.49687264
CGTACTA807.102972E-834.49687246
CGCGGCA901.9949402E-730.66861237
CCGTATG450.004625296730.66388964
TACGAAC802.7578917E-630.19406728
GGTATCA37100.029.2906991
TATATCG2600.029.1896666
CAACGCG953.204259E-729.0535834
TATCAAC106750.028.8910122
GCGATCT605.4250704E-428.7482811
ACGAACG854.423193E-628.4170729
ATCAACG105150.028.4119933
TCAACGC106550.027.8767934
GTGTCGG5700.027.8395865
GCGTGTA2850.027.8395859
GTCGGAA5600.027.72070167
CAACGCA108600.027.445875
Produced by FastQC (version 0.11.5)