FastQCFastQC Report
Mon 6 Feb 2017
SRR2079279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622671
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95710.5898299778574955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49790.3068397722027447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44890.27664264659934146No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAA25490.15708667992464276No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23970.14771940830889319No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC20530.12651979359956517No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGCGTTCTATCATTCTAGCCTCG17170.10581319318580293No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.10248534669073399No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAG16420.10119118416487384No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAGTATTAAGTAGTGGGACTT16260.1002051555737423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131350.040.0051041
GGTATCA54050.037.3278851
TAACGCA754.0305167E-836.79158423
TTGCGGC400.002599561834.4921165
TTAACGC802.7675014E-630.180622
TATCAAC176400.029.63172
ATCAACG180500.029.2265853
TCAACGC182750.028.8649654
CAACGCA186500.028.227265
CTCGTAT9850.028.01389739
AACGCAG190350.027.6166366
CGACGAA500.00771656827.6141115
TATGCCG11400.027.53317643
TCTCGTA10050.027.45640838
TGCCGTC11850.027.36083245
AATCTCG10050.027.11320336
ATGCCGT12000.027.0188244
GCCGTCT11950.026.84323346
CCGTCTT12150.026.68525747
TATATCG2700.025.54971366
Produced by FastQC (version 0.11.5)