FastQCFastQC Report
Mon 6 Feb 2017
SRR2079278_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079278_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498588
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62900.039.9832651
GGTATCA26900.030.6511651
ATCAACG84900.029.5005653
TCAACGC84900.029.2567584
TATCAAC86150.029.1526132
CAACGCA86350.028.8054265
AACGCAG88950.028.1185826
ACGCAGA102400.024.1557527
CGCAGAG106000.023.1719768
AGAGTAC104200.022.38040511
GAGTACT50850.022.04956212
CAGAGTA114100.020.89206310
GTACTTT54950.020.59419314
AGTACTT55450.020.59368313
GCAGAGT118850.020.260279
GTACATG111050.019.1365431
TACATGG111200.018.6763952
ACATGGG118400.017.1327483
TACTTTT66450.016.87434815
GTATATA14550.016.3601571
Produced by FastQC (version 0.11.5)