FastQCFastQC Report
Mon 6 Feb 2017
SRR2079278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498588
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83050.043.4364471
GGTATCA31250.037.2626151
TATCAAC115200.031.2542212
TCAACGC115050.031.1990644
ATCAACG115000.031.1282963
CAACGCA116850.030.5984485
AACGCAG121350.029.5190166
ACGCAGA137550.025.8130597
CGCAGAG141200.025.066748
AGAGTAC142750.023.53388211
GAGTACT96000.023.53318612
AGTACTT100650.022.54876713
CAGAGTA152750.022.35448510
GCAGAGT155150.022.2087639
GTACTTT103950.021.53430714
TACTTTT114950.019.51923615
ACTTTTT121250.018.96054616
GTACATG104850.018.71861
TATACCG1852.6106864E-718.6730825
TACATGG105650.018.347922
Produced by FastQC (version 0.11.5)