FastQCFastQC Report
Mon 6 Feb 2017
SRR2079277_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079277_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2204553
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46000.038.248721
GGTATCA19700.028.544731
TCAACGC59800.028.0375234
ATCAACG60350.027.8963323
TATCAAC61000.027.8252942
CAACGCA60100.027.7827635
AACGCAG62900.026.4911636
ACGCAGA70600.023.4064397
CGCAGAG71850.022.8071698
AGAGTAC67150.022.24571811
ACTCGAC1808.594725E-921.08262646
GCAGAGT77850.020.0301579
TATATCG1901.6010745E-819.97301566
CAGAGTA76700.019.97064810
GTACATG79600.019.8931791
TTAGTCG2303.45608E-1019.49978839
GAGTACT31400.019.3373712
TACATGG80800.018.9145892
GCGTTAG1108.2418567E-418.817551
GTACTTT32600.018.62598814
Produced by FastQC (version 0.11.5)