FastQCFastQC Report
Mon 6 Feb 2017
SRR2079277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2204553
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57700.039.5120351
GGTATCA24350.033.479141
TATCTCG13500.029.63839536
CGCTATC13800.029.49397733
ATCAACG77700.029.1638013
TCAACGC77800.029.1203614
TATCAAC78000.028.9187722
CAACGCA79600.028.5052475
CTCGTAT14050.027.9871739
CGCTACG14950.027.91737728
TACGCTA15200.027.68513731
ATGCGCG756.174618E-527.59436651
AACGCAG82800.027.4023536
TATGCCG14750.027.36051643
TCTCGTA14400.027.30692538
GCCGTCT14600.027.16911146
ACGCTAT15150.026.865832
GCTACGC16100.025.9232829
ACGCTAC16350.025.73786427
TCGTATG15850.025.46167840
Produced by FastQC (version 0.11.5)