FastQCFastQC Report
Mon 6 Feb 2017
SRR2079276_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079276_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4194935
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77400.036.7708851
ATCAACG99650.028.2836783
TCAACGC99550.028.2081284
GGTATCA37350.028.1708771
TATCAAC101100.027.809782
CAACGCA101600.027.6389685
AACGCAG107200.026.2916816
ACGCAGA124050.022.4145167
CGCAGAG126800.021.819578
AGAGTAC123150.020.95408211
GTACATG135200.020.3618551
TACATGG134700.019.8483872
GAGTACT62900.019.69021412
CAGAGTA138600.018.9414210
GCAGAGT140800.018.7924679
ACATGGG139450.018.4796283
GTACTTT66200.018.4494314
AGTACTT68500.018.28195813
CATGGGG95750.016.1770254
TATACCG2603.3096512E-815.922055
Produced by FastQC (version 0.11.5)