FastQCFastQC Report
Mon 6 Feb 2017
SRR2079276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4194935
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100650.039.8489651
GGTATCA43500.033.831321
ATCAACG135450.029.3558673
TCAACGC135450.029.3520134
TATCAAC137150.029.1431272
CAACGCA137950.028.8180165
AACGCAG142600.027.8036446
ACGCAGA162300.024.2145967
CGCAGAG165600.023.7476968
AGAGTAC163300.023.00234211
GAGTACT109700.022.76471912
AGTACTT113800.022.00516913
CAGAGTA177800.021.35924310
GCAGAGT182950.021.07859
GTACTTT117450.020.93952814
GTACATG121950.019.404721
TACATGG121950.018.7815042
TACTTTT135050.018.24705715
ACTTTTT141100.017.53805416
ACATGGG128000.017.2460443
Produced by FastQC (version 0.11.5)