FastQCFastQC Report
Mon 6 Feb 2017
SRR2079275_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079275_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651268
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42840.25943699024022754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31810.192639837991168No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAA28640.17344246966573565No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC19760.1196656145459126No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAG17770.10761426976117748No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACAATTATTAGGG17140.10379901990470353No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGC17140.10379901990470353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCG803.45608E-1143.12441334
GTATCAA95300.032.9056931
CGCGGCA1056.4392225E-1032.85669337
ACGCGGC1056.4392225E-1032.85669336
CGATTAC450.00462015430.67089325
GTCACCG450.004624947430.66439260
AACGCGG1151.7043931E-929.99959235
TGTTTCG3700.029.83562366
GTCGGAA5950.029.56923567
GTGTCGG6100.028.84212565
TTAGTCG4800.027.3121339
TGTGTCG6450.027.27704664
TAGTCGG4900.026.75473840
AGTCGGC5100.025.70553241
CCCAACG1359.416908E-925.55985332
TCGGCCT5400.025.55520843
TATCAAC123750.024.8946652
TGGTCGG5150.024.78531810
GTACTTT58900.024.71857814
ATCAACG121300.024.5727063