FastQCFastQC Report
Mon 6 Feb 2017
SRR2079275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651268
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96840.5864584065094218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47980.2905645843073323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44270.26809700181920804No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAA28690.17374526727339232No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20840.12620604287129647No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC20370.12335974535932386No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAG17920.10852266258414744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16790.1016794366511069No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGC16780.10161887712957557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA138350.038.1199871
GGTATCA61750.032.1816441
CGCGGCA953.2132266E-729.0443637
TATCAAC186800.028.1403662
TATCTCG8250.028.01020436
ATCAACG189150.027.809023
GACCGTC500.00771122127.61806320
TCAACGC192600.027.3931224
CTCGTAT8650.027.1136639
CAACGCA195750.026.9161975
TAGGGCG3250.026.53090331
AACGCAG198550.026.5018166
TGCCGTC9650.026.44842345
CGGACTC9450.026.27822728
TCTCGTA8800.026.25956538
TATGCCG9650.026.09101343
ACGGACT9550.026.00306527
TATATCG3500.025.62127166
GCCGTCT10000.025.52272846
ATGCCGT10100.025.2700344
Produced by FastQC (version 0.11.5)