##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2079275_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1651268
Sequences flagged as poor quality	0
Sequence length	75
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.817073909262458	31.0	31.0	34.0	27.0	34.0
2	30.865947865519104	31.0	31.0	34.0	27.0	34.0
3	30.927819106286805	31.0	31.0	34.0	27.0	34.0
4	34.38850810407517	37.0	35.0	37.0	30.0	37.0
5	34.578645622636664	37.0	35.0	37.0	31.0	37.0
6	34.48003170896548	37.0	35.0	37.0	31.0	37.0
7	34.35127913821378	37.0	35.0	37.0	30.0	37.0
8	34.26751018005557	37.0	35.0	37.0	30.0	37.0
9	35.75241087455216	39.0	37.0	39.0	30.0	39.0
10	35.75446626471294	39.0	37.0	39.0	30.0	39.0
11	35.70028123841799	39.0	37.0	39.0	30.0	39.0
12	35.6959512326285	39.0	37.0	39.0	30.0	39.0
13	35.629170431450255	39.0	37.0	39.0	30.0	39.0
14	36.77529147297713	40.0	37.0	41.0	30.0	41.0
15	36.73692822727746	40.0	37.0	41.0	30.0	41.0
16	36.80512793804519	40.0	37.0	41.0	30.0	41.0
17	36.942638626800736	40.0	37.0	41.0	30.0	41.0
18	37.00199361944881	40.0	37.0	41.0	30.0	41.0
19	37.123526889638754	40.0	37.0	41.0	30.0	41.0
20	37.109180944583194	40.0	37.0	41.0	30.0	41.0
21	37.09797501071903	40.0	37.0	41.0	30.0	41.0
22	37.06368923760407	40.0	37.0	41.0	30.0	41.0
23	37.06042750177439	40.0	37.0	41.0	30.0	41.0
24	37.05894742706817	40.0	37.0	41.0	30.0	41.0
25	37.04281860969873	40.0	37.0	41.0	30.0	41.0
26	36.963713945888856	40.0	37.0	41.0	30.0	41.0
27	36.890817844226376	40.0	36.0	41.0	30.0	41.0
28	36.82424597339741	40.0	36.0	41.0	30.0	41.0
29	36.76320621486034	40.0	36.0	41.0	30.0	41.0
30	36.6084257673497	40.0	36.0	41.0	30.0	41.0
31	36.43685034773277	40.0	36.0	41.0	30.0	41.0
32	36.24977592977034	40.0	35.0	41.0	30.0	41.0
33	36.10353013562911	40.0	35.0	41.0	30.0	41.0
34	36.02810385715704	40.0	35.0	41.0	29.0	41.0
35	35.944466918755765	40.0	35.0	41.0	29.0	41.0
36	35.80601392384519	40.0	35.0	41.0	28.0	41.0
37	35.714206900394124	40.0	35.0	41.0	27.0	41.0
38	35.638743075018716	39.0	35.0	41.0	27.0	41.0
39	35.55025834691885	39.0	35.0	41.0	27.0	41.0
40	35.45850401025152	39.0	35.0	41.0	27.0	41.0
41	35.36287992015833	39.0	35.0	41.0	26.0	41.0
42	35.230018385870736	39.0	35.0	41.0	25.0	41.0
43	35.043502932292036	39.0	35.0	41.0	24.0	41.0
44	34.88121492089715	39.0	35.0	41.0	24.0	41.0
45	34.6587216611719	39.0	35.0	41.0	22.0	41.0
46	34.374650268763155	39.0	34.0	41.0	18.0	41.0
47	34.08370960982712	39.0	34.0	41.0	13.0	41.0
48	33.83885474677642	39.0	33.0	41.0	8.0	41.0
49	33.69136929922944	39.0	33.0	41.0	2.0	41.0
50	33.5481956896155	39.0	33.0	41.0	2.0	41.0
51	33.46159375704004	39.0	33.0	41.0	2.0	41.0
52	33.39969950365416	39.0	33.0	41.0	2.0	41.0
53	33.33662978995535	39.0	33.0	41.0	2.0	41.0
54	33.228987662814276	39.0	33.0	41.0	2.0	41.0
55	33.16568661174322	39.0	33.0	41.0	2.0	41.0
56	33.081917653585	39.0	33.0	41.0	2.0	41.0
57	32.98962130919996	39.0	33.0	41.0	2.0	41.0
58	32.90365767398145	39.0	33.0	41.0	2.0	41.0
59	32.80254446885666	39.0	33.0	41.0	2.0	41.0
60	32.692280114433274	38.0	32.0	41.0	2.0	41.0
61	32.569711882020364	38.0	32.0	41.0	2.0	41.0
62	32.44398486496438	38.0	32.0	40.0	2.0	41.0
63	32.31352451570551	38.0	32.0	40.0	2.0	41.0
64	32.17890736088872	38.0	32.0	40.0	2.0	41.0
65	32.056522623826055	37.0	32.0	40.0	2.0	41.0
66	31.90423359503121	37.0	32.0	40.0	2.0	41.0
67	31.708295685497447	37.0	31.0	40.0	2.0	41.0
68	31.513704014127324	37.0	31.0	40.0	2.0	41.0
69	31.345892368773573	37.0	31.0	40.0	2.0	41.0
70	31.157184660515433	36.0	31.0	39.0	2.0	41.0
71	30.91137295702454	36.0	31.0	39.0	2.0	41.0
72	30.716907249459204	36.0	31.0	39.0	2.0	41.0
73	30.46780716394916	36.0	31.0	39.0	2.0	41.0
74	30.21342144339986	35.0	30.0	39.0	2.0	40.0
75	30.005650808954087	35.0	30.0	38.0	2.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.49201696677438633
1101	2	0.5712348785857841
1101	3	0.5873248118987284
1101	4	0.5903376288481468
1101	5	0.45959996308965856
1101	6	0.5833994404969403
1101	7	0.38157369471221614
1101	8	0.6226853101258598
1101	9	0.7104383021491003
1101	10	0.7689788938793285
1101	11	0.7468110991519694
1101	12	0.6786863073020939
1101	13	0.7645392440484713
1101	14	0.863066592850636
1101	15	0.8327744050036614
1101	16	0.6679616750899271
1101	17	0.801878022698773
1101	18	0.7976466546952352
1101	19	0.8122805796863588
1101	20	0.9446887608738876
1101	21	1.0725386243911927
1101	22	0.9619870390331045
1101	23	0.8814549295080383
1101	24	0.872307853777265
1101	25	0.9219739135372222
1101	26	0.9787604583954419
1101	27	1.0026140318969752
1101	28	0.9099974818481442
1101	29	0.9289718062793
1101	30	0.9645698442709403
1101	31	1.0284234546800946
1101	32	1.0368206104603885
1101	33	0.9718061019903885
1101	34	0.9852145545161193
1101	35	1.0772165630041783
1101	36	1.053603308063046
1101	37	1.0725696300579202
1101	38	1.1107595516219249
1101	39	1.096082989672425
1101	40	1.17891199286953
1101	41	1.0916208182130163
1101	42	1.2463767844174782
1101	43	1.1745651133088728
1101	44	1.2934764489004635
1101	45	1.065263265449687
1101	46	1.0070111789722773
1101	47	1.0542578160392821
1101	48	1.1931567461099846
1101	49	1.1831344056990218
1101	50	1.1926834575974112
1101	51	1.2351315506911789
1101	52	1.3734591241432241
1101	53	1.3811898651448686
1101	54	1.3496941883219904
1101	55	1.2313931275282073
1101	56	1.310890488832115
1101	57	1.295788172654106
1101	58	1.2949082160777508
1101	59	1.2860322413797505
1101	60	1.2805930395283838
1101	61	1.3099172387994713
1101	62	1.1906532270115093
1101	63	1.3112832652462743
1101	64	1.2825928219478442
1101	65	1.4131645332860359
1101	66	1.5047539179646243
1101	67	1.3775030004344337
1101	68	1.4570946478556017
1101	69	1.4595284891840485
1101	70	1.4104602954374634
1101	71	1.4145834303676388
1101	72	1.6698693698787146
1101	73	1.4841318597313808
1101	74	1.435113749045417
1101	75	1.590442747966403
1102	1	0.392194856597893
1102	2	0.46276879247943015
1102	3	0.4205324260853125
1102	4	0.29087886731282
1102	5	0.32772814714396503
1102	6	0.28609341166387026
1102	7	0.4412240145668349
1102	8	0.5040287733579021
1102	9	0.5723756028168765
1102	10	0.42281131561039587
1102	11	0.44464079161451053
1102	12	0.4571780319915604
1102	13	0.5541743742240968
1102	14	0.6611717967002662
1102	15	0.6260902816519547
1102	16	0.5514984851391915
1102	17	0.5942654976010999
1102	18	0.672076857123372
1102	19	0.573790804474406
1102	20	0.6010813487240654
1102	21	0.6260661818066282
1102	22	0.5984534190379662
1102	23	0.7025848289548762
1102	24	0.798412133101948
1102	25	0.7482771281813427
1102	26	0.6575962215605031
1102	27	0.7072718801316284
1102	28	0.6903519175371642
1102	29	0.6971473611604111
1102	30	0.7101628435790204
1102	31	0.644528871199995
1102	32	0.6645926161109159
1102	33	0.6734535212587573
1102	34	0.6842399634448739
1102	35	0.6918089191323702
1102	36	0.5291279501234669
1102	37	0.7788775190174491
1102	38	0.8136335171619535
1102	39	0.7453012787253002
1102	40	0.6262563466583444
1102	41	0.6867569825716942
1102	42	0.6828153252926299
1102	43	0.5231582772887293
1102	44	0.5667427984816626
1102	45	0.5916559788344031
1102	46	0.49689235684068933
1102	47	0.5157956953474283
1102	48	0.5184608535262001
1102	49	0.350943805872852
1102	50	0.30594348614544487
1102	51	0.5944787784824612
1102	52	0.596183607587065
1102	53	0.7337000759025898
1102	54	0.6264965504389153
1102	55	0.5852227431931496
1102	56	0.7820550210424315
1102	57	0.7343626467567006
1102	58	0.4678774549470788
1102	59	0.5478367338916144
1102	60	0.6637370003202534
1102	61	0.6241964601013521
1102	62	0.5222355381614108
1102	63	0.671211274881415
1102	64	0.6413878823853381
1102	65	0.6454084114466454
1102	66	0.7064882883609194
1102	67	0.6702312646831565
1102	68	0.8080921674408366
1102	69	0.8362112981153125
1102	70	0.7851543050031928
1102	71	0.5992607955306255
1102	72	0.7580640281626287
1102	73	0.7686651186301177
1102	74	0.8389184793344633
1102	75	0.8897080386268534
1103	1	0.4708688357135635
1103	2	0.3861983204831603
1103	3	0.4039997184068085
1103	4	0.3228389767687574
1103	5	0.4633284454503439
1103	6	0.27513339308463003
1103	7	0.3364821260335944
1103	8	0.2545877371904126
1103	9	0.36579014337745264
1103	10	0.3504406454163629
1103	11	0.3521654545412858
1103	12	0.4036653590192998
1103	13	0.4928542386879826
1103	14	0.5902955433591046
1103	15	0.36046706222222724
1103	16	0.45354999059107115
1103	17	0.4220213335441301
1103	18	0.5841020804564678
1103	19	0.5712635667077777
1103	20	0.4088111333462834
1103	21	0.41370824877554213
1103	22	0.48761918535213766
1103	23	0.5462978201398343
1103	24	0.30626323090691443
1103	25	0.40640006271982543
1103	26	0.5639492241699671
1103	27	0.4533948348649304
1103	28	0.6071946878628935
1103	29	0.6553321784044854
1103	30	0.5908774373673822
1103	31	0.6358133628746785
1103	32	0.646292500856184
1103	33	0.7266412739645034
1103	34	0.5812074223736516
1103	35	0.6823822645633584
1103	36	0.7407664426088303
1103	37	0.543755693751713
1103	38	0.7100567977531469
1103	39	0.662098250078941
1103	40	0.6301939763920501
1103	41	0.7913160358384275
1103	42	0.7082081506492486
1103	43	0.5343916472131554
1103	44	0.3886378084853064
1103	45	0.26017203443940673
1103	46	0.31332719175522783
1103	47	0.3593114263927788
1103	48	0.402753537086312
1103	49	0.3793189737423148
1103	50	0.22034087985937134
1103	51	0.41792518358771247
1103	52	0.3281651379795534
1103	53	0.22483382765340565
1103	54	0.15750750324506413
1103	55	0.1497917207725692
1103	56	0.24890485955597086
1103	57	0.41627832760350003
1103	58	0.3322086384934906
1103	59	0.25481058303917337
1103	60	0.30465502809390443
1103	61	0.19445100141201976
1103	62	0.35726006765811036
1103	63	0.4412279500347154
1103	64	0.3697119862040381
1103	65	0.39859414467015597
1103	66	0.3484268759061955
1103	67	0.31854023680997656
1103	68	0.4165318721925324
1103	69	0.44879779326062064
1103	70	0.3186611214769748
1103	71	0.2506177433428789
1103	72	0.3639014858075953
1103	73	0.42547575033858465
1103	74	0.4772890745191525
1103	75	0.5460004567693062
1104	1	0.409389811518178
1104	2	0.39058181777274825
1104	3	0.452241748219933
1104	4	0.37652750020600934
1104	5	0.42802698067444567
1104	6	0.32651658937360395
1104	7	0.3121299103033621
1104	8	0.4376196713866136
1104	9	0.4389723247035988
1104	10	0.4869317658981913
1104	11	0.44904264347684375
1104	12	0.5970150035853976
1104	13	0.209837354522584
1104	14	0.4562423168856782
1104	15	0.533004061386734
1104	16	0.5822513135872356
1104	17	0.6218389749933237
1104	18	0.4532294037170601
1104	19	0.5307153376885054
1104	20	0.5261366725452845
1104	21	0.5262392279277321
1104	22	0.4888358467208178
1104	23	0.641627458010781
1104	24	0.7662769165891277
1104	25	0.6214730905328452
1104	26	0.6657342000195854
1104	27	0.6295709174122379
1104	28	0.7053898094024404
1104	29	0.6871791688202862
1104	30	0.6896619012346505
1104	31	0.6292476111186076
1104	32	0.789726327136627
1104	33	0.8567048357301132
1104	34	0.8040853680303854
1104	35	0.7425936401361781
1104	36	0.704418860037606
1104	37	0.6507491054952368
1104	38	0.8487280628840495
1104	39	0.7559344888652646
1104	40	0.6211555186404425
1104	41	0.8468812418550584
1104	42	0.8162002092965466
1104	43	0.8984547054356753
1104	44	0.6619896191306012
1104	45	0.7473897057073486
1104	46	0.7232552188509089
1104	47	0.7280361261657617
1104	48	0.8491430768929433
1104	49	0.9036054334968426
1104	50	0.8432120741004141
1104	51	0.8729797434657343
1104	52	0.7725725043366864
1104	53	0.8051130830847768
1104	54	0.9610620532772742
1104	55	0.8809250302884024
1104	56	0.8365744537114992
1104	57	0.8671667013843773
1104	58	0.7310186311513291
1104	59	0.8107203925648605
1104	60	0.8642858522448407
1104	61	0.8351146374432048
1104	62	0.8504770047096031
1104	63	0.7808684652802569
1104	64	0.8207332784812564
1104	65	0.7871229971264029
1104	66	0.8080407511906706
1104	67	0.8473891766970603
1104	68	0.9464427535054618
1104	69	0.7954471219167267
1104	70	0.6550103215748706
1104	71	0.7046922930375175
1104	72	0.6363650633265046
1104	73	0.6297591719560565
1104	74	0.6707255732349786
1104	75	0.707807276261768
1105	1	0.20886451692081565
1105	2	0.354544610659719
1105	3	0.21589543964937974
1105	4	0.10498754826109291
1105	5	0.2228514811040938
1105	6	0.21557666917350105
1105	7	0.05772121222392457
1105	8	0.17829394768692453
1105	9	0.22175226745346777
1105	10	0.23055543658404076
1105	11	0.2486493908921048
1105	12	0.02443636675155858
1105	13	0.12739593191699328
1105	14	-0.024012271061145896
1105	15	0.20480987616121382
1105	16	0.21023149497054305
1105	17	0.153242396865501
1105	18	0.15272778090020012
1105	19	0.18171187859827143
1105	20	0.1830600526018742
1105	21	0.13985898524408924
1105	22	0.15943139040551557
1105	23	0.23153301718475916
1105	24	0.23161828282030683
1105	25	0.21788496856707695
1105	26	0.15737450389409702
1105	27	0.21902151317485163
1105	28	0.23590998651662431
1105	29	0.2001658086315672
1105	30	0.17879751180239367
1105	31	0.14450291058715692
1105	32	0.08198524441949218
1105	33	0.2146502072812808
1105	34	0.12072416676277697
1105	35	0.166707111431748
1105	36	0.2605120858034482
1105	37	0.330753839851333
1105	38	0.24732800705696434
1105	39	0.2956059300251539
1105	40	0.20092257737382369
1105	41	0.16826671148676553
1105	42	0.2258909983741404
1105	43	0.2322268105000731
1105	44	-0.007884140495463043
1105	45	0.08767839196458027
1105	46	-0.015006725135911836
1105	47	-0.21082431705753635
1105	48	-0.29879186812831904
1105	49	-0.19789792981162435
1105	50	-0.2108095287938383
1105	51	-0.08419759511461677
1105	52	-0.06746578100541711
1105	53	-0.0922403297565424
1105	54	-0.3084931635452932
1105	55	-0.2067590759582103
1105	56	-0.2851977006120876
1105	57	-0.38381848254398676
1105	58	-0.29618407591517837
1105	59	-0.25912435886775853
1105	60	-0.3257349187163143
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2215	1	-0.5039192020845391
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2215	47	-1.0062378079523029
2215	48	-0.7681537352494487
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2215	59	-1.0124365664346584
2215	60	-1.1294597258992276
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2215	62	-1.2276000918511407
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2215	68	-1.2186244129120212
2215	69	-1.2783689655106656
2215	70	-1.1994553808505373
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2215	75	-1.423176396357185
2216	1	-0.9077943316751806
2216	2	-1.0692407861262048
2216	3	-0.9995483112160244
2216	4	-0.9623506247405658
2216	5	-1.152834413306735
2216	6	-0.9742655376700426
2216	7	-0.9007018169864622
2216	8	-1.0169931686035838
2216	9	-1.2693642378124252
2216	10	-1.3123690210726267
2216	11	-1.213432354101272
2216	12	-1.2080280775107752
2216	13	-1.2615873703795373
2216	14	-1.6772821555703459
2216	15	-1.763886663070906
2216	16	-1.7211776510418133
2216	17	-1.579850789827205
2216	18	-1.4115441071073462
2216	19	-1.2124040812865502
2216	20	-1.3058692767489504
2216	21	-1.5611970194580564
2216	22	-1.4905735547596706
2216	23	-1.4790863718188945
2216	24	-1.7787597821224281
2216	25	-1.6525125661974371
2216	26	-1.4708310228225088
2216	27	-1.4943171515175138
2216	28	-1.7540404487992092
2216	29	-1.5948190874210582
2216	30	-1.6882801216393588
2216	31	-1.5655000282538651
2216	32	-1.3592863977427072
2216	33	-2.1342673667054513
2216	34	-1.9448152377263028
2216	35	-1.9834100183378638
2216	36	-2.294182571440082
2216	37	-1.989214737156189
2216	38	-1.769300464095771
2216	39	-2.0726352301388573
2216	40	-1.8759125734254596
2216	41	-1.89910113250599
2216	42	-1.9183500035445817
2216	43	-1.9584556505532191
2216	44	-1.8815252797484874
2216	45	-2.1011692715155164
2216	46	-2.2864789149398277
2216	47	-1.7466199658773363
2216	48	-1.6834868644688967
2216	49	-1.7744774862161776
2216	50	-1.7581494537968752
2216	51	-1.756565283687891
2216	52	-2.024198835337245
2216	53	-2.511978885937861
2216	54	-2.3992191420521927
2216	55	-1.9532908196799674
2216	56	-2.178211736403785
2216	57	-2.2281636155124183
2216	58	-2.6438599895697656
2216	59	-2.246219738387996
2216	60	-2.569415845355497
2216	61	-2.2433439596858626
2216	62	-2.3054551938509427
2216	63	-2.0563821515886147
2216	64	-2.1348686886608554
2216	65	-2.1515039638897626
2216	66	-2.008781960980876
2216	67	-2.0404429779845934
2216	68	-2.3332626880472844
2216	69	-2.039728080239609
2216	70	-2.182325773659425
2216	71	-2.663361165132219
2216	72	-2.256681414110055
2216	73	-2.3275057767854186
2216	74	-2.441812393405666
2216	75	-2.3678943586544356
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17764.0
3	8299.0
4	4816.0
5	3116.0
6	2307.0
7	2365.0
8	2729.0
9	3374.0
10	4159.0
11	4560.0
12	4848.0
13	5104.0
14	5631.0
15	6473.0
16	7890.0
17	9213.0
18	10140.0
19	11493.0
20	14176.0
21	17203.0
22	24006.0
23	29308.0
24	25779.0
25	22660.0
26	20191.0
27	18173.0
28	19625.0
29	22924.0
30	26404.0
31	28228.0
32	34059.0
33	42300.0
34	53818.0
35	74013.0
36	98198.0
37	131069.0
38	229031.0
39	474861.0
40	130961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.414496011549915	21.229382510894656	15.634530554700993	20.721590922854435
2	13.160068504930756	23.57212760133425	41.82876431929887	21.43903957443613
3	15.763625570886889	32.47271251529432	30.49608102050953	21.267580893309265
4	9.507645934777969	19.771640444642752	42.61966021300949	28.101053407569793
5	9.98683742677448	41.63589039386562	34.29507545310326	14.082196726256639
6	24.301282400760837	44.03832058201731	18.982499500245336	12.677897516976513
7	23.692300602597392	36.59084376974449	23.472071812048796	16.244783815609324
8	24.92426233757739	34.55636081289133	21.267293079618728	19.252083769912556
9	27.6379121983833	16.31927706465577	21.243432319889926	34.799378417071004
10	13.974170152876455	31.125353364808134	33.76593018213882	21.13454630017659
11	28.77697623886613	28.7740088223111	25.55048605071981	16.898528888102962
12	22.98354961157123	30.03461582250731	30.20684710174242	16.77498746417904
13	25.999898260003828	25.838931051773546	28.3563298023095	19.804840885913126
14	22.204511926592172	23.69324664439691	31.52874033772834	22.573501091282576
15	21.418691575201603	31.356569618014763	27.774837276565645	19.44990153021799
16	19.458743220361566	31.30921207217726	29.2711419345618	19.960902772899374
17	17.29761613499444	30.404816177628348	29.70159901360651	22.595968673770702
18	17.153242235663743	28.59608494805204	34.988626921856415	19.2620458944278
19	18.944653442082085	27.74183233733107	35.96902501592715	17.34448920465969
20	19.027801665144604	27.353464125750637	35.82943531879743	17.78929889030733
21	20.59999878800366	26.508238545119596	35.074931673706345	17.816830993170402
22	18.96893781021615	27.076585993309383	35.41296748922646	18.541508707248006
23	19.227648086198002	27.500078727377993	34.79737995286047	18.47489323356354
24	18.72046209337309	27.44272886048782	35.56715203104523	18.269657015093856
25	18.655663405334565	27.63367303187611	35.639944575925895	18.07071898686343
26	18.548351933181046	27.18056669177868	36.366477155737286	17.904604219302982
27	18.271958276912045	28.193121891782557	35.051790502813596	18.483129328491803
28	18.111414985332484	26.605311796752556	36.26007407640674	19.023199141508222
29	18.514014684472784	27.155071133213994	36.01075052626224	18.320163656050987
30	18.37727128485503	27.658381316660897	35.44518515468113	18.519162243802946
31	19.075704246675887	26.68409973426482	36.015534728463216	18.224661290596075
32	18.13939348427996	27.50237998919618	35.789647713151346	18.56857881337251
33	18.581901908109405	27.12921221752011	35.86716390071145	18.42172197365903
34	18.464355876817088	26.732183994360696	36.249233922052625	18.554226206769584
35	18.495544030405725	27.794337442498733	35.3844439545852	18.325674572510337
36	18.45938999605152	27.134904812544058	35.2665345661637	19.139170625240723
37	19.387464663519186	26.575879869288325	35.36863791946552	18.668017547726958
38	18.344569143228114	27.624891901254067	36.18909831717201	17.841440638345805
39	18.8524818503114	28.082661324509406	34.29746110261932	18.767395722559876
40	18.566762027726572	27.01929668594074	36.529200590091975	17.88474069624071
41	18.682430713851417	27.574143022210812	35.00951995678472	18.73390630715305
42	18.92751509748872	27.393009493310593	34.420820847978646	19.258654561222045
43	18.422630366482	27.21175484536732	34.939997626066756	19.425617162083924
44	18.348263274041525	28.040148540394412	34.705147801568245	18.906440383995815
45	19.485934445529132	26.985565032447788	34.01846338692447	19.510037135098603
46	19.156369529355622	27.31282868680311	33.49971052548708	20.031091258354188
47	19.385647877873247	27.827705738862495	33.20066760816536	19.585978775098894
48	18.768728032033565	28.295649161735103	32.84379034778122	20.091832458450114
49	19.87539272849713	27.935501687188268	32.58980371447881	19.599301869835788
50	19.589127870218523	27.380837029482798	32.758159184335916	20.271875915962763
51	19.79236562447767	28.574767996473017	31.24865255064593	20.384213828403386
52	19.411930710217845	28.38406606317085	32.17121630165424	20.032786924957062
53	19.81362201653517	28.63908220833929	31.72525598509751	19.822039790028025
54	19.471521279404673	28.665183362119294	31.791689780217386	20.071605578258648
55	20.32141360457539	28.034879862021185	31.72222800902095	19.921478524382476
56	19.811744671367702	28.949328637144305	31.320173345574432	19.918753345913565
57	19.91681544122456	28.412892395419764	30.97050266825252	20.699789495103158
58	19.45438293481131	27.985160494843964	31.798048529978175	20.76240804036655
59	19.349372724475977	29.037261062407797	31.706119176293612	19.90724703682261
60	19.580165061031884	29.841188710736233	30.82849059026154	19.750155637970334
61	20.705179292519446	29.086193155805113	31.0174362974393	19.191191254236138
62	19.56944602572084	29.392321537146	31.59281231150849	19.445420125624672
63	19.56351119261077	28.88010910403399	31.871204431988026	19.685175271367218
64	19.93032021452605	28.154000440873318	31.519535290455575	20.396144054145058
65	19.285240191174296	29.101393595709478	31.458309614187403	20.155056598928823
66	19.905127453569015	29.036534348149424	30.782101996768546	20.276236201513022
67	19.716969020171167	28.92746664987149	31.03021435648241	20.32534997347493
68	19.710125794238127	28.50682021331486	31.801318744140865	19.98173524830615
69	19.69389584246773	29.235956852552096	30.584980754184055	20.485166550796116
70	20.03811616285182	28.03221524307381	31.782726971030744	20.146941623043624
71	19.97137957012429	28.5246852721666	31.620124655719117	19.883810501989984
72	20.002325485626805	29.390444191978528	30.28333377743649	20.323896544958178
73	19.985489938641095	27.804753680202122	31.5147510882546	20.695005292902184
74	19.4363967569165	29.158925141164243	30.528660399159918	20.876017702759334
75	19.761601387539756	28.719020776760644	30.908610837247497	20.6107669984521
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2373.0
1	1759.5
2	1922.0
3	2624.0
4	2550.0
5	1933.5
6	1456.5
7	2403.5
8	3211.0
9	5756.5
10	10879.0
11	11547.0
12	9638.0
13	8057.0
14	6369.5
15	6684.0
16	7105.0
17	7542.5
18	8516.0
19	10021.5
20	10991.0
21	11931.5
22	13322.0
23	15018.0
24	16264.0
25	18098.5
26	21852.5
27	27779.5
28	31787.0
29	35275.5
30	42558.5
31	51056.0
32	55759.0
33	66758.0
34	85396.0
35	98390.5
36	103746.0
37	102806.5
38	97100.0
39	90904.0
40	89475.0
41	90413.0
42	85442.5
43	77311.0
44	75088.0
45	71568.5
46	65377.0
47	63480.0
48	64255.0
49	58862.5
50	48109.0
51	39706.5
52	36665.0
53	34423.5
54	30178.5
55	26274.0
56	24373.0
57	22391.0
58	18828.0
59	15518.0
60	13789.0
61	11820.0
62	8099.0
63	5177.0
64	4007.0
65	3454.0
66	2302.5
67	1588.5
68	1473.0
69	1277.0
70	879.0
71	539.0
72	401.0
73	377.0
74	322.5
75	242.5
76	193.0
77	157.0
78	105.0
79	69.5
80	50.0
81	39.5
82	20.0
83	7.0
84	3.0
85	2.5
86	1.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.01986352306227699
4	0.10682699598127014
5	0.02270982057424961
6	0.027736260861350184
7	0.12560044765598316
8	0.010537356746451818
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.06649435464140285
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	1651268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.17638939121478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.60534178444198	35.166765762902486
2	10.015560519389195	8.849026029516287
3	3.8220606158632773	5.065345141296068
4	1.9675308761157666	3.4767364049011227
5	1.135761605551225	2.50869234712111
6	0.6971012700818495	1.84772502913477
7	0.4849702547241182	1.4996964371094486
8	0.33929149161857425	1.1990938440694578
9	0.2521695630913515	1.0025946730562443
>10	1.3294317069192425	10.99335998751586
>50	0.15973054110314563	5.037383229242963
>100	0.17353118859205044	15.701522805056134
>500	0.013242393077063954	4.039554560800042
>1k	0.004138247836582486	3.0223838754949535
>5k	1.3794159455274952E-4	0.5901198727830382
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9684	0.5864584065094218	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4798	0.2905645843073323	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4427	0.26809700181920804	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAA	2869	0.17374526727339232	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2084	0.12620604287129647	No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGACGTAATTCGTGAAGGAACC	2037	0.12335974535932386	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGAGCCGTAATTACAG	1792	0.10852266258414744	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1679	0.1016794366511069	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGTGAGCTTTTTAGTTTGTGTCGGAAGC	1678	0.10161887712957557	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.055952153133229E-5	0.0
3	0.0	0.0	0.0	6.055952153133229E-5	0.0
4	0.0	0.0	0.0	6.055952153133229E-5	0.0
5	0.0	0.0	0.0	6.055952153133229E-5	0.0
6	0.0	0.0	0.0	6.055952153133229E-5	0.0
7	0.0	0.0	0.0	6.055952153133229E-5	0.0
8	0.0	0.0	0.0	6.055952153133229E-5	0.0
9	0.0	0.0	0.0	6.055952153133229E-5	0.0
10	0.0	0.0	0.0	6.055952153133229E-5	0.0
11	0.0	0.0	0.0	6.055952153133229E-5	0.0
12	0.0	0.0	0.0	6.055952153133229E-5	0.0
13	0.0	0.0	0.0	6.055952153133229E-5	0.0
14	0.0	0.0	0.0	6.055952153133229E-5	0.0
15	0.0	0.0	0.0	6.055952153133229E-5	0.0
16	0.0	0.0	0.0	1.2111904306266458E-4	0.0
17	0.0	0.0	0.0	1.2111904306266458E-4	0.0
18	0.0	0.0	0.0	1.8167856459399686E-4	0.0
19	0.0	0.0	0.0	1.8167856459399686E-4	0.0
20	0.0	0.0	0.0	2.4223808612532915E-4	0.0
21	0.0	0.0	0.0	6.055952153133228E-4	0.0
22	0.0	0.0	0.0	7.267142583759874E-4	0.0
23	0.0	0.0	0.0	0.001090071387563981	0.0
24	0.0	0.0	0.0	0.002180142775127962	0.0
25	0.0	0.0	0.0	0.002301261818190627	0.0
26	0.0	0.0	0.0	0.0032096546411606113	0.0
27	0.0	0.0	0.0	0.003815249856473934	0.0
28	0.0	0.0	0.0	0.005510916459351238	0.0
29	0.0	0.0	0.0	0.008296654449792523	0.0
30	0.0	0.0	0.0	0.013444213779955767	0.0
31	0.0	0.0	0.0	0.01992408258380832	0.0
32	0.0	0.0	0.0	0.02652507043072354	0.0
33	0.0	0.0	0.0	0.032399344019262775	0.0
34	0.0	0.0	0.0	0.03778914143555134	0.0
35	0.0	0.0	0.0	0.046933629186782524	0.0
36	0.0	0.0	0.0	0.05638091454567036	0.0
37	0.0	0.0	0.0	0.06909841406725013	0.0
38	0.0	0.0	0.0	0.08563116344530385	0.0
39	0.0	0.0	0.0	0.10385957942623487	0.0
40	0.0	0.0	0.0	0.12348086440238654	0.0
41	0.0	0.0	0.0	0.14437389933069616	0.0
42	0.0	0.0	0.0	0.16435854143603582	0.0
43	0.0	0.0	0.0	0.18597829062272145	0.0
44	0.0	0.0	0.0	0.21111049205822435	0.0
45	0.0	0.0	0.0	0.2344864673693186	0.0
46	0.0	0.0	0.0	0.26307056153210745	0.0
47	0.0	0.0	0.0	0.29123073904417696	0.0
48	0.0	0.0	0.0	0.3201781903361538	0.0
49	0.0	0.0	0.0	0.3511241058386646	0.0
50	0.0	0.0	0.0	0.38352344985792736	0.0
51	0.0	0.0	0.0	0.4183451747384434	0.0
52	0.0	0.0	0.0	0.4512895544514882	0.0
53	0.0	0.0	0.0	0.48253826756165563	0.0
54	0.0	0.0	0.0	0.5198429328249563	0.0
55	0.0	0.0	0.0	0.555512491006911	0.0
56	0.0	0.0	0.0	0.5957240133037157	0.0
57	0.0	0.0	0.0	0.6329075595239537	0.0
58	0.0	0.0	0.0	0.6771765697633576	0.0
59	0.0	0.0	0.0	0.7244129965577968	0.0
60	0.0	0.0	0.0	0.7740112446919579	0.0
61	0.0	0.0	0.0	0.8243362070844951	0.0
62	0.0	0.0	0.0	0.8762357170368469	0.0
63	0.0	0.0	0.0	0.9275901912954166	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	13835	0.0	38.119987	1
GGTATCA	6175	0.0	32.181644	1
CGCGGCA	95	3.2132266E-7	29.04436	37
TATCAAC	18680	0.0	28.140366	2
TATCTCG	825	0.0	28.010204	36
ATCAACG	18915	0.0	27.80902	3
GACCGTC	50	0.007711221	27.618063	20
TCAACGC	19260	0.0	27.393122	4
CTCGTAT	865	0.0	27.11366	39
CAACGCA	19575	0.0	26.916197	5
TAGGGCG	325	0.0	26.530903	31
AACGCAG	19855	0.0	26.501816	6
TGCCGTC	965	0.0	26.448423	45
CGGACTC	945	0.0	26.278227	28
TCTCGTA	880	0.0	26.259565	38
TATGCCG	965	0.0	26.091013	43
ACGGACT	955	0.0	26.003065	27
TATATCG	350	0.0	25.621271	66
GCCGTCT	1000	0.0	25.522728	46
ATGCCGT	1010	0.0	25.27003	44
>>END_MODULE