FastQCFastQC Report
Mon 6 Feb 2017
SRR2079274_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079274_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4658257
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71700.040.0801241
GGTATCA31950.031.8532941
TCAACGC96300.029.3758724
ATCAACG96350.029.3248213
TATCAAC98700.029.0810012
CAACGCA97150.028.976815
AACGCAG99200.028.2388866
ACGCAGA117500.023.6059467
CGCAGAG121550.022.7058738
AGAGTAC118550.021.85453611
GTACATG143500.020.9636941
TACATGG145400.020.0965822
CAGAGTA131000.020.06720410
GAGTACT55800.020.03195812
GCAGAGT139050.019.2279939
AGTACTT60350.019.09312813
ACATGGG153000.018.466973
GTACTTT61450.018.13437314
CATGGGG109800.015.9925854
TACTTTT73150.015.51684615
Produced by FastQC (version 0.11.5)