FastQCFastQC Report
Mon 6 Feb 2017
SRR2079274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4658257
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100800.042.1945571
GGTATCA39800.035.071731
TATCAAC139100.030.5518052
ATCAACG140050.030.1965433
TCAACGC141000.029.987934
CAACGCA142300.029.6168695
AACGCAG146650.028.7368146
ACGCAGA168000.024.8979177
TATGCCG13950.024.23086543
CGCAGAG173800.024.0355158
GAGTACT113150.023.16733712
TCGTATG15500.022.92042240
AGAGTAC176500.022.76660511
AGTACTT117600.022.37867213
TCTCGTA14650.022.36675838
ACGTAGA15750.022.3376127
CTCGTAT15000.021.84486639
CAGAGTA187500.021.65171210
GTACTTT122450.021.37962514
GACGTAG16400.021.03164326
Produced by FastQC (version 0.11.5)