FastQCFastQC Report
Mon 6 Feb 2017
SRR2079273_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079273_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3544611
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79950.039.3532451
GGTATCA32750.035.0781821
TCAACGC102400.029.782184
ATCAACG102950.029.6230723
TATCAAC106500.029.1863232
CAACGCA105700.028.8197275
AACGCAG109950.027.7057346
ACGCAGA124850.023.8465987
CGCAGAG127600.023.1160458
GAGTACT58950.022.29757312
AGAGTAC124700.022.24270811
CAGAGTA136400.020.71417210
AGTACTT64050.020.41439613
GTACTTT64300.020.0136614
GCAGAGT142800.019.9790749
GTACATG137650.019.42361
TACATGG139200.018.835562
ACATGGG138650.017.8403553
TACTTTT80200.015.95982915
ACTTTTT84500.015.55618516
Produced by FastQC (version 0.11.5)