FastQCFastQC Report
Mon 6 Feb 2017
SRR2079273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3544611
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37590.10604830826288132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108000.042.2570651
GGTATCA41150.038.4517331
TATCAAC147600.030.826182
ATCAACG147800.030.807843
TCAACGC148550.030.6488324
CAACGCA152000.029.8841615
AACGCAG158200.028.7966526
ACGCAGA180750.024.9330027
TATGCCG14550.024.89165543
CGCAGAG183550.024.375298
CTCGTAT14000.023.65215139
GAGTACT120600.023.62436912
AGAGTAC181900.023.4755311
TCGTATG15850.022.63244840
TCTCGTA14200.022.59030238
AGTACTT127400.022.5258613
CAGAGTA192100.022.49837510
AATCTCG14700.022.29121436
CCGTCTT16200.022.14347547
TATATCG2057.4578566E-1121.87343466
Produced by FastQC (version 0.11.5)