FastQCFastQC Report
Mon 6 Feb 2017
SRR2079272_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079272_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026400
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57850.036.7351461
TATCAAC75950.027.93522
TCAACGC75250.027.9199894
ATCAACG76750.027.419273
CAACGCA76950.027.3480035
AACGCAG79250.026.4672456
GGTATCA29250.023.7068921
ACGCAGA90600.022.8088347
CGCAGAG93900.021.6758698
AGAGTAC86200.021.21123111
GAGTACT41200.020.85002312
ACGCGTT1004.3385313E-420.69895711
AGTACTT45050.019.98711613
CAGAGTA96400.019.50336510
GTACATG91950.019.4349061
ATCGCGC900.00573327319.16855435
GTACTTT44800.019.09851614
GTATACG1108.242839E-418.8175471
GCAGAGT102700.018.8108189
TACATGG94950.018.3848462
Produced by FastQC (version 0.11.5)