FastQCFastQC Report
Mon 6 Feb 2017
SRR2079272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026400
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72300.039.6640321
GGTATCA33450.030.0575731
TATCAAC99050.028.9521392
ATCAACG101650.028.0756443
TCAACGC102250.027.8026224
CAACGCA103500.027.4330165
AACGCAG107850.026.3247956
ACGCAGA122450.022.9020657
CGCAGAG125450.022.2453028
AGAGTAC120650.021.95626411
GAGTACT78550.021.60446212
ACCGTCG1304.9319715E-621.2279018
AGTACTT82150.020.7416813
GTACATG94150.020.7340981
CAGAGTA133150.020.17998510
GTACTTT84600.020.10023514
TACATGG95750.019.9185332
GCAGAGT137650.019.7457949
CCGCGTA900.005743753619.16249741
TATGCCG14500.018.31670243
Produced by FastQC (version 0.11.5)