FastQCFastQC Report
Mon 6 Feb 2017
SRR2079271_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079271_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456752
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25850.037.7690771
GGTATCA10050.031.2381841
TCAACGC32500.029.8286384
ATCAACG32850.029.5108283
TATCAAC33050.029.019092
CAACGCA33700.028.868865
AACGCAG34250.028.6067316
TATATCG500.00773633727.5994515
GGACCGT1101.1533339E-625.0904086
ACGCAGA39400.024.4297187
CGCAGAG40450.023.7939388
AGAGTAC38550.023.3567111
CAGAGTA43050.021.63571510
GCAGAGT44750.021.199249
GAGTACT20200.020.4946412
GCGATAC1056.0206786E-419.71592314
TTAGTCG1056.023464E-419.71457139
GTACATG40600.019.628921
AGTACTT21600.019.48572213
ACGTCCC900.00572783919.1708924
Produced by FastQC (version 0.11.5)