FastQCFastQC Report
Mon 6 Feb 2017
SRR2079271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1456752
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32550.044.481471
GGTATCA11450.037.4224551
TATCAAC44350.032.3348272
ATCAACG45150.032.144573
TCAACGC45350.031.9951064
CAACGCA45700.031.597795
AACGCAG47400.030.4593016
ACGCAGA54550.026.210957
CGCAGAG55750.025.5540838
AGAGTAC53600.025.16154511
GAGTACT37500.024.65067912
TACGCTA7450.023.6123531
CAGAGTA57650.023.573410
CGCTATC7250.023.31220833
AGTACTT39550.023.28574213
CTCGTAT7450.023.14936339
TATGCCG7750.023.14338943
CGCTACG7750.023.14338928
GCAGAGT60550.023.0140239
TATCTCG7400.022.83966336
Produced by FastQC (version 0.11.5)