FastQCFastQC Report
Mon 6 Feb 2017
SRR2079270_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079270_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3747843
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62100.039.7756231
TCAACGC80850.030.16724
ATCAACG81750.029.7506833
TATCAAC82650.029.6771582
GGTATCA25000.029.6683941
CAACGCA83250.029.1317585
AACGCAG86800.028.139036
ACGCAGA101550.023.8820157
CGCAGAG104550.022.8331628
AGAGTAC101650.022.05950411
GTACATG113450.020.8296361
CAGAGTA111050.020.40942810
TATATCG2201.9099389E-1020.38444366
GAGTACT50850.020.15042712
TACATGG114450.020.0749282
GCAGAGT114700.020.0005659
AGTACTT53700.019.33797513
ACATGGG122500.018.2488083
GTACTTT54750.018.148914
CATGGGG88600.016.54824
Produced by FastQC (version 0.11.5)