FastQCFastQC Report
Mon 6 Feb 2017
SRR2079270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3747843
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89500.041.5720631
GGTATCA35400.033.4733961
TCAACGC119550.030.945854
ATCAACG119950.030.8171483
TATCAAC120650.030.6383512
CAACGCA123900.029.9143315
AACGCAG128300.028.9134226
ACGCAGA145850.025.2911937
CGCAGAG149050.024.717178
GAGTACT95650.023.90855212
AGAGTAC148950.023.5739311
AGTACTT100150.023.04092213
GTACTTT102700.022.26731114
CAGAGTA160750.022.14386410
GCAGAGT165750.021.6423559
TACTTTT116350.019.75679615
ACTTTTT120550.019.32614716
GTACATG115050.019.0676021
TACATGG116700.018.2947522
ACATGGG120350.017.4241473
Produced by FastQC (version 0.11.5)