FastQCFastQC Report
Mon 6 Feb 2017
SRR2079269_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079269_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3485053
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74050.038.9012151
GGTATCA33250.031.2303311
CGCGAAA605.4256996E-428.74885212
TCAACGC101700.027.8838424
TATCAAC102600.027.8409942
ATCAACG101650.027.8296813
CAACGCA105300.026.9633125
AACGCAG109550.025.8227926
ACGCAGA125400.022.3663357
CGCAGAG128450.021.539828
GAGTACT55850.021.37246712
AGAGTAC124100.020.8214911
GTACTTT59250.019.62284514
AGTACTT62250.019.45223413
CAGAGTA135550.019.18993810
GCAGAGT139550.019.1095929
GTACATG137750.018.6330131
TACATGG137800.018.2507232
ACATGGG140500.017.0651553
TACTTTT69650.016.59373515
Produced by FastQC (version 0.11.5)