FastQCFastQC Report
Mon 6 Feb 2017
SRR2079269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3485053
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38440.11029961380788184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98050.042.466481
GGTATCA37550.037.7294731
TATCAAC135950.030.4497992
ATCAACG138000.030.0725843
TCAACGC138800.029.817714
CAACGCA140250.029.4087945
AACGCAG145050.028.550576
ACGCAGA166800.024.6809797
CGCAGAG170150.024.0236288
GAGTACT111300.023.11843512
AGAGTAC168750.023.05579611
CTCGTAT17300.022.32989739
AGTACTT117150.022.1406513
CAGAGTA179550.021.9571310
TATGCCG17950.021.90560243
GTACTTT118300.021.77963614
GCAGAGT183050.021.7634149
TATCTCG16650.021.54437436
ACGGACT18600.021.14008327
TCGTATG19100.020.94784740
Produced by FastQC (version 0.11.5)