FastQCFastQC Report
Mon 6 Feb 2017
SRR2079268_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079268_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4251992
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70650.040.2363781
GGTATCA29700.032.2917941
TCAACGC92950.030.2119564
ATCAACG93450.030.0872293
TATCAAC94750.029.9657022
CAACGCA96250.029.1044355
AACGCAG99150.028.3227586
ACGCAGA114900.024.2602467
CGCAGAG118250.023.3392878
AGAGTAC118400.022.02794311
GAGTACT58950.021.94682712
CAGAGTA127100.020.7370310
GCAGAGT131850.020.303949
AGTACTT67000.019.56739613
GTACATG128550.019.4030531
GTACTTT66850.019.25049414
TACATGG125800.018.9770762
ACATGGG130850.017.6646463
CATGGGG91350.015.974814
ACTTTTT85400.015.83662616
Produced by FastQC (version 0.11.5)