FastQCFastQC Report
Mon 6 Feb 2017
SRR2079268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4251992
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97850.042.6264231
GGTATCA37250.036.9223441
TATCAAC132300.031.239482
ATCAACG133350.031.0971583
TCAACGC133850.030.9231644
CAACGCA137200.030.0659715
AACGCAG143050.028.8571826
ACGCAGA165200.024.8172257
CGCAGAG169150.024.2077588
AGAGTAC171150.023.13553611
GAGTACT114800.022.8041812
CAGAGTA181400.022.09446110
GCAGAGT185650.021.7001369
TATGCCG15000.021.61479843
AGTACTT121550.021.59455513
GTACTTT123100.021.26661114
CTCGTAT15100.020.55796639
CGTATGC16250.019.9521241
TCGTATG16150.019.86209140
TCTCGTA15700.019.33292838
Produced by FastQC (version 0.11.5)