FastQCFastQC Report
Mon 6 Feb 2017
SRR2079267_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079267_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5054622
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78700.040.5479131
GGTATCA30450.033.6488841
TCAACGC102450.030.5419544
TATCAAC103450.030.4134582
ATCAACG102800.030.404413
CAACGCA105000.029.7016535
AACGCAG109800.028.4346376
ACGCAGA128150.024.1745957
CGCAGAG131100.023.5512088
AGAGTAC130000.022.50370211
GAGTACT64850.022.39660512
CAGAGTA144200.020.45493310
GTACATG149950.020.15391
GCAGAGT149400.019.927719
GTACTTT72000.019.69392614
AGTACTT74600.019.51548813
TACATGG152650.019.1872332
ACATGGG155500.018.48063
ACTTTTT93550.015.78421416
CATGGGG111850.015.6685654
Produced by FastQC (version 0.11.5)