FastQCFastQC Report
Mon 6 Feb 2017
SRR2079267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5054622
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109950.043.0856361
GGTATCA40300.039.09761
ATCAACG153550.030.4465623
TCAACGC154050.030.3880834
TATCAAC154900.030.2927462
CAACGCA158250.029.447965
AACGCAG163500.028.5198386
ACGCAGA190900.024.2613647
CGCAGAG195400.023.5492788
AGAGTAC199300.022.22131511
GAGTACT135250.021.93174612
CAGAGTA210950.021.20666910
AGTACTT144200.020.76187113
GCAGAGT219800.020.5568059
GTACTTT145950.020.1584414
GTACATG146500.019.6706491
TACATGG146800.019.0420072
ACATGGG151600.017.7781123
TACTTTT167150.017.61581415
ACTTTTT173350.017.26455316
Produced by FastQC (version 0.11.5)