FastQCFastQC Report
Mon 6 Feb 2017
SRR2079266_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079266_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726044
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49650.037.86891
ATCAACG63000.029.2419343
TCAACGC63600.029.2372864
CAACGCA65050.028.6916435
TATCAAC64850.028.620532
GGTATCA22150.028.0352361
AACGCAG69700.026.9259826
ACGCAGA79500.023.2162517
CGCAGAG80850.022.5719688
AGAGTAC81600.020.75853711
GTACATG96350.020.7315731
TATACCG2900.020.2235015
TACATGG97450.020.0373362
GAGTACT38650.019.99419412
CAGAGTA91750.018.95039410
ACATGGG100450.018.6489893
GCAGAGT97000.018.1025529
GTACTTT45800.017.02393714
CATGGGG73850.016.4903414
AGTACTT48000.016.31511113
Produced by FastQC (version 0.11.5)