FastQCFastQC Report
Mon 6 Feb 2017
SRR2079266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726044
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66500.041.358181
GGTATCA26600.031.3044011
ATCAACG89800.030.4347783
TCAACGC89700.030.4207844
TATCAAC90200.030.2232022
CAACGCA92100.029.6264675
AACGCAG96000.028.4546646
ACGCAGA110650.024.3416797
CGCAGAG113600.023.624978
GAGTACT75700.022.28138712
AGAGTAC114950.021.90499111
CAGAGTA123550.020.65942210
GCAGAGT128100.020.4372239
AGTACTT85500.019.96955113
GTACATG94050.019.9485361
GTACTTT87600.019.37270414
TACATGG94950.018.9952722
CGCCGAT950.007847019518.15413767
ACATGGG98050.017.9013633
CTCGTAT11350.017.01850339
Produced by FastQC (version 0.11.5)