FastQCFastQC Report
Mon 6 Feb 2017
SRR2079265_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079265_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759804
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29900.034.4980961
GGTATCA14600.028.5908871
TCAACGC38900.026.0731124
ATCAACG39250.025.9285053
TATCAAC40350.025.6491432
CAACGCA39550.025.6446045
AACGCAG41100.024.677476
ACGCAGA46750.021.3260967
CGCAGAG47500.020.9161478
AGAGTAC45550.020.44834711
GTACATG47950.019.7131981
TACATGG47650.019.185722
GCAGAGT51750.018.4651229
CAGAGTA51500.018.42079210
ACATGGG49100.017.9165273
GAGTACT24850.017.49298712
GTATTAT5500.016.9354291
TATACTG7250.016.6542555
CATGGGG31550.016.5109754
TATACAG9700.016.3599225
Produced by FastQC (version 0.11.5)