FastQCFastQC Report
Mon 6 Feb 2017
SRR2079265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1759804
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42450.042.815631
GGTATCA16850.036.296741
TATCAAC56900.031.8209652
TCAACGC58050.031.3637924
CAACGCA58150.031.1901445
ATCAACG58400.031.1811493
AACGCAG61350.029.727986
ACGCAGA67550.026.8948637
CGCAGAG68350.026.548358
AGAGTAC64900.026.1478911
GAGTACT46300.026.07373812
TATCTCG6050.025.65508336
TACGCTA6600.025.08496731
TATGCCG6700.024.71056643
ACGCTAC7500.024.37422627
CGCTATC6300.024.08953333
GTACTTT50000.023.9373314
CGCTACG7250.023.78747228
CAGAGTA72300.023.71015210
ACGCTAT6900.023.49443432
Produced by FastQC (version 0.11.5)