FastQCFastQC Report
Mon 6 Feb 2017
SRR2079264_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079264_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3430324
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60200.042.0630571
TCAACGC79700.030.6461724
ATCAACG81300.030.2976513
TATCAAC83800.029.9289632
CAACGCA82050.029.8104765
GGTATCA24900.028.6795481
AACGCAG85750.028.4437336
ACGCAGA99600.024.176727
CGCAGAG102850.023.278248
AGAGTAC101750.022.07231311
GAGTACT53600.021.30478512
CAGAGTA111650.020.4859510
GCAGAGT114450.020.1053359
AGTACTT57650.019.92777813
GTACATG109050.019.2344251
GTACTTT59550.019.00312614
TAGTCGG1852.6463567E-718.6489740
TACATGG111550.018.4013062
TTAGTCG2105.0446033E-818.07174339
ACATGGG115000.017.0093043
Produced by FastQC (version 0.11.5)