FastQCFastQC Report
Mon 6 Feb 2017
SRR2079264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3430324
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78550.040.781551
GGTATCA32000.038.8761021
ATCAACG107400.029.7302043
TCAACGC108950.029.3629594
TATCAAC110450.028.9092252
CAACGCA112000.028.4995735
AACGCAG115400.027.7169326
ACGCAGA133250.023.7941997
CGCAGAG137200.022.9041988
GAGTACT91800.021.79205312
AGAGTAC137700.021.59166711
AGTACTT97600.020.70907413
GCAGAGT148000.020.6483279
CAGAGTA147200.020.61995510
GTACATG105850.020.2409571
GCGCGTT1206.40385E-520.12007536
GTACTTT101250.019.65593114
TACATGG108100.019.4999892
ACATGGG111400.017.9917343
TACTTTT113500.017.45628415
Produced by FastQC (version 0.11.5)