FastQCFastQC Report
Mon 6 Feb 2017
SRR2079263_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079263_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3585123
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49200.038.6357041
GGTATCA22100.031.8449271
TCAACGC63900.029.261784
ATCAACG64500.029.096553
CAACGCA65600.028.5034735
TATCAAC67400.027.9981782
AACGCAG68950.027.168646
ACGCAGA79850.023.0279167
CGCAGAG83300.022.0741778
AGAGTAC80800.021.092311
GAGTACT41450.020.64180212
GTACATG90000.019.242591
CAGAGTA90050.019.23190710
GCAGAGT93750.018.8776729
TACATGG91350.018.6938612
ACATGGG92850.017.1657333
GTATTAG9250.017.1560941
GTACTTT48550.017.05511514
AGTACTT50400.016.90779313
GTATTAT12400.016.1364731
Produced by FastQC (version 0.11.5)