FastQCFastQC Report
Mon 6 Feb 2017
SRR2079263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3585123
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68400.042.5752941
GGTATCA26400.038.2089541
TATCAAC93150.030.7438242
ATCAACG94500.030.6336293
TCAACGC94350.030.52824
CAACGCA96900.029.7596365
AACGCAG101200.028.4915646
ACGCAGA115150.024.7993227
CGCAGAG117650.024.191028
AGAGTAC119500.022.65861311
GAGTACT82450.022.38178412
CAGAGTA129300.021.23469710
GCAGAGT131350.021.1396319
AGTACTT90200.020.49697713
GTACTTT92850.019.83768314
TATGCCG6650.019.1916243
GTACATG89150.018.2122141
TATCTCG6850.017.62418436
TACATGG89000.017.6218722
TACTTTT106700.017.17696615
Produced by FastQC (version 0.11.5)