FastQCFastQC Report
Mon 6 Feb 2017
SRR2079262_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079262_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3820981
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69350.038.453011
GGTATCA30100.033.0083121
TCAACGC89950.029.0713624
ATCAACG90750.028.7770733
TATCAAC92400.028.3752042
CAACGCA92850.028.0890625
AACGCAG96300.027.0827566
ACGCAGA110400.023.3113337
CGCAGAG113800.022.584258
AGAGTAC110400.021.59295311
GAGTACT54150.021.27982712
CAGAGTA122000.019.93521710
GCAGAGT124650.019.8435129
AGTACTT59750.019.57410213
GTACATG121900.019.187721
GTACTTT60150.018.9279714
TACATGG122100.018.7042222
ACATGGG123600.017.8352723
TACTTTT73200.015.64778115
GTATAGG10800.015.3325651
Produced by FastQC (version 0.11.5)