FastQCFastQC Report
Mon 6 Feb 2017
SRR2079262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3820981
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90900.042.8017581
GGTATCA33350.038.5419961
TATCAAC127300.030.1830732
ATCAACG129150.029.9112423
TCAACGC129750.029.7142124
CAACGCA132400.029.1436615
AACGCAG137550.027.9749556
ACGCAGA154500.024.8374847
CGCAGAG158350.024.094238
GAGTACT110500.023.31695612
AGAGTAC159950.022.90096311
AGTACTT116500.022.0272613
CAGAGTA170050.021.82474310
GCAGAGT175300.021.2301489
GTACTTT122400.020.74005514
GTACATG113800.020.0092241
TACATGG113700.019.3886362
TACTTTT134950.018.92788315
ACATGGG116600.018.5211753
GCACCGT6300.018.0933176
Produced by FastQC (version 0.11.5)