FastQCFastQC Report
Mon 6 Feb 2017
SRR2079261_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079261_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4327770
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79500.038.5338251
TCAACGC103950.028.7733784
ATCAACG104900.028.5785753
TATCAAC106750.028.406472
CAACGCA106150.028.209545
GGTATCA35050.028.1497341
AACGCAG109950.027.014956
ACGCAGA128050.022.980837
GAGTACT63100.022.63483212
CGCAGAG132600.022.0101578
AGAGTAC127450.021.76293611
GTACTTT69200.020.3413814
AGTACTT70450.020.17541513
CAGAGTA140550.019.95519410
GTACATG136500.019.7385221
GCAGAGT149700.019.1502959
TACATGG140650.018.7882042
ACATGGG143350.017.7123583
TACTTTT81900.017.43985215
CATGGGG99550.016.1834774
Produced by FastQC (version 0.11.5)