FastQCFastQC Report
Mon 6 Feb 2017
SRR2079261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4327770
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA108050.041.1528241
GGTATCA42600.036.3340871
ATCAACG149050.029.7167913
TATCAAC150500.029.5223082
TCAACGC150150.029.515954
CAACGCA152050.029.0549015
AACGCAG157750.028.0256566
ACGCAGA180150.024.327217
CGCAGAG184650.023.669568
GAGTACT122500.023.34243612
AGAGTAC182150.022.89416911
TATGCCG10500.022.33815843
AGTACTT129100.022.25596813
CAGAGTA196200.021.64147210
GTACTTT133550.021.28193314
GCAGAGT204100.021.0066059
TCGTATG12000.020.40820740
TACTTTT145700.019.25793515
GTACATG129100.018.3320141
ACTTTTT156550.018.23186716
Produced by FastQC (version 0.11.5)