FastQCFastQC Report
Mon 6 Feb 2017
SRR2079260_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079260_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890119
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19680.10412042839630734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75600.042.6676181
GGTATCA28200.039.270331
TATCAAC103900.030.9131132
ATCAACG104450.030.387013
TCAACGC106300.029.858174
CAACGCA108600.029.1940465
AACGCAG112700.027.9176946
GTATCGA658.660391E-426.537821
GAGTACT52550.025.66921812
GTACTTT54350.024.24908814
AGTACTT57150.024.14639713
TGTGTCG4500.023.76546564
ACGCAGA132850.023.6573147
CGCAGAG135600.023.0751618
GTGTCGG4800.022.99883765
AGAGTAC132350.022.99014711
GTACATG159650.022.6032731
CAGAGTA137900.022.11490610
GCAGAGT139750.021.9208959
TACATGG163900.021.8908672
Produced by FastQC (version 0.11.5)