FastQCFastQC Report
Mon 6 Feb 2017
SRR2079260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890119
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59320.3138426733978125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31650.1674497743263784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24660.1304679758258607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA112750.045.327621
GGTATCA38600.044.491841
TATCAAC154650.032.8904042
ATCAACG156850.032.5171973
TCAACGC158000.032.228244
CAACGCA161650.031.4997045
AACGCAG166450.030.627166
ACGCAGA192050.026.3806487
CGCAGAG193850.026.122378
GAGTACT124400.025.53625112
AGAGTAC195050.025.18151711
AGTACTT129550.025.02696813
GCAGAGT200650.024.719389
CAGAGTA200600.024.57079110
GTACTTT130550.024.0426514
TATCCGC750.00199527822.9964648
TACTTTT136850.022.97683515
GTACATG146750.022.7462751
ACTTTTT140550.022.61754216
TAGTCGG3250.022.28711140
Produced by FastQC (version 0.11.5)