FastQCFastQC Report
Mon 6 Feb 2017
SRR2079259_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079259_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2613351
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35650.037.5458151
GGTATCA14700.032.150861
ATCAACG43250.030.1505373
TCAACGC43850.029.8953294
CAACGCA44550.029.270725
TATCAAC45750.028.578372
AACGCAG47100.027.4662726
ACGCAGA54700.023.4609157
CGCAGAG56250.022.6304518
AGAGTAC57850.020.335611
GTACATG63400.018.9356121
CAGAGTA64300.018.6169210
TACATGG64250.018.2555582
GCAGAGT67250.018.0567559
GAGTACT30500.017.30636412
ACATGGG64000.017.2488173
CATGGGG43750.015.6126454
GTATTAT10950.015.4372991
TATACTG9200.015.3739465
CGAATTA1350.003246413515.33198966
Produced by FastQC (version 0.11.5)