FastQCFastQC Report
Mon 6 Feb 2017
SRR2079259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2613351
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51650.042.475351
GGTATCA18850.035.740191
ATCAACG68250.032.0937273
TCAACGC68300.031.8642354
TATCAAC69300.031.4080452
CAACGCA70000.031.1866995
AACGCAG73200.029.86716
ACGCAGA84400.025.7794467
CGCAGAG86500.025.0838688
AGAGTAC86750.023.6175811
TACGCTA5750.023.39457531
GAGTACT60650.022.52074812
CAGAGTA92950.022.30198310
ACGCTAT6050.022.23451232
TTAGTCG1403.787991E-722.1734339
GCAGAGT95850.022.0230629
CGCTACG6350.021.72724528
AGTACTT65250.020.88021713
CGCTATC5800.020.81414233
TATGCCG6550.020.53722443
Produced by FastQC (version 0.11.5)