FastQCFastQC Report
Mon 6 Feb 2017
SRR2079257_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079257_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4287213
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70100.036.8613471
TATCAAC91550.027.7726042
ATCAACG91550.027.6595543
TCAACGC92000.027.561764
GGTATCA32200.027.3206961
CAACGCA93550.026.9944655
AACGCAG97000.026.0343516
GTACATG134200.022.2367341
ACGCAGA112650.022.141887
CGCAGAG115700.021.349478
TACATGG136650.021.2068942
AGAGTAC108200.021.1394511
ACATGGG138950.020.1606673
GAGTACT51550.020.01106512
CAGAGTA124950.018.88544710
GCAGAGT128650.018.6372769
CATGGGG97800.017.2495374
GTACTTT58800.017.13319214
AGTACTT62000.016.86081713
GTATAGG11650.015.9909861
Produced by FastQC (version 0.11.5)