FastQCFastQC Report
Mon 6 Feb 2017
SRR2079257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4287213
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98250.040.6876071
GGTATCA41950.034.4275171
TATCAAC133700.029.8994582
TCAACGC134150.029.7928944
ATCAACG134200.029.7623123
CAACGCA136300.029.2972565
AACGCAG141600.028.2708916
ACGCAGA161500.024.5492347
CGCAGAG165000.023.8959338
GAGTACT104400.023.19304812
AGAGTAC163850.022.88254511
AGTACTT110900.022.11359213
TATCTCG11200.021.8656136
CAGAGTA178100.021.47775310
CTCGTAT11450.021.38819139
GCAGAGT181100.021.2171949
TATGCCG12650.020.9952743
GTACTTT115250.020.91979614
CGTATAC1901.5746991E-820.0032443
GTACATG131600.019.795971
Produced by FastQC (version 0.11.5)