FastQCFastQC Report
Mon 6 Feb 2017
SRR2079256_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079256_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3423675
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47200.038.3727951
GGTATCA20950.029.641121
TCAACGC62550.028.4595834
TATCAAC63350.028.1001872
CAACGCA63500.028.0881375
ATCAACG63550.028.066043
AACGCAG66100.027.0355016
ACGCAGA77300.023.0736987
CGCAGAG80150.022.0810628
AGAGTAC79550.020.38279711
CGTGCCG4300.020.0572510
CAGAGTA87650.019.16800310
GTACATG91350.018.9206181
GCAGAGT89350.018.9191369
TACATGG90650.018.4578062
GAGTACT35500.017.58987412
ACATGGG97450.016.745013
TCGATCG1907.0252263E-616.34257341
TATACCG1907.029128E-616.3416185
AGTACTT38500.015.95017213
Produced by FastQC (version 0.11.5)