FastQCFastQC Report
Mon 6 Feb 2017
SRR2079256_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079256_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3423675
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67900.042.5488971
GGTATCA26450.032.9250641
TATCAAC90900.031.1746622
ATCAACG91800.030.9819643
TCAACGC92000.030.9467414
CAACGCA93000.030.4644785
AACGCAG97050.029.3304186
ACGCAGA109050.025.9425757
CGCAGAG113850.024.782598
AGAGTAC112700.024.17745211
GAGTACT72100.023.2970612
CAGAGTA123250.022.47170310
AGTACTT77900.021.7838713
GCAGAGT128350.021.7400259
GTACTTT77800.021.3685414
GCGATAC3150.019.70921514
GTACATG90300.019.0587041
TACTTTT89900.018.58330715
TACATGG92500.018.1197322
ACTTTTT93500.017.94163516
Produced by FastQC (version 0.11.5)