FastQCFastQC Report
Mon 6 Feb 2017
SRR2079255_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079255_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4227823
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA66850.041.387821
TCAACGC85400.031.7111034
CAACGCA86600.031.1920175
ATCAACG86850.031.1816753
GGTATCA25800.030.7544331
TATCAAC89350.030.4636572
AACGCAG90300.029.9521436
ACGCAGA103300.025.7486027
CGCAGAG106650.024.6804458
AGAGTAC109000.022.85126711
GAGTACT58750.021.37511812
CAGAGTA119300.021.25378210
GCAGAGT122900.020.8838429
GTACATG116600.019.290731
AGTACTT66900.018.87426213
GTACTTT67500.018.70759614
TACATGG118050.018.4400862
CGAATCG2651.1641532E-1018.22688937
ACATGGG120200.017.4501323
TATACTG13900.016.380565
Produced by FastQC (version 0.11.5)