FastQCFastQC Report
Mon 6 Feb 2017
SRR2079255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2079255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4227823
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98050.042.8820461
GGTATCA36850.040.127191
ATCAACG132600.031.3961243
TATCAAC132900.031.325252
TCAACGC133650.031.1428224
CAACGCA134800.030.6447075
AACGCAG140500.029.4485446
ACGCAGA158350.025.9090127
CGCAGAG162050.025.2044478
AGAGTAC167500.023.37272511
GAGTACT115400.023.31401412
AGTACTT121600.022.40896413
GCAGAGT180950.022.2644779
CAGAGTA178600.022.15186110
GTACTTT127800.021.15989514
CGAATCG3200.020.48009137
TACTTTT139100.019.40304615
GTACATG118500.019.3298151
TACATGG117650.018.764692
ACTTTTT149000.018.48446516
Produced by FastQC (version 0.11.5)